Purpose: The expansion of radiopharmaceutical therapy (RPT) development demands scalable preclinical dosimetry methods. While PET and SPECT remain the gold standards, their low throughput and high cost limit large-cohort studies. Cherenkov luminescence imaging (CLI) offers a high-throughput alternative but suffers from depth-dependent attenuation and photon scatter that compromise quantitative accuracy. This work develops and validates a quantitative CLI methodology incorporating attenuation and...